Composition, Conservation, Evolution, and Function of the Cold Shock Domain Proteins in Plants

Composition, Conservation, Evolution, and Function of the Cold Shock Domain Proteins in Plants

4.11 - 1251 ratings - Source



Cold shock domain proteins, or CSPs, are among the most ancient and conserved nucleic acid binding proteins. They have been well characterized in prokaryotic systems but little is known about their function in plants. The overall objective of this study was to understand the evolution and function of the cold shock domain in plant model systems. To pinpoint conserved areas within CSD an alignment was made of the cold shock domain protein sequences based on nucleotide sequence information found within EST databases. A description of domains found in the plant CSPs was prepared and a descriptive nomenclature system was developed. The alignment was also used to analyze the phylogeny of CSPs by making a neighbor-joining tree and a maximum likelihood tree. Prediction software was used to predict sub-cellular localization and post-translation modifications by phosphorylation and sumoylation. To characterize the CSPs on a functional level, their expression in the cold response pathways of ICE1, CBF 1, 2, and 3, and ABA were analyzed by qRT-PCR. The characteristics of nucleic acid binding, cold induction, and localization of the CSPs in bryophytes, the earliest land plants, were also determined. Physcomitrella patens CSPs are capable of binding to nucleic acids and are cold inducible. Expression lines for two CSPs from Physcomitrella patens were created in order to view sub-cellular and tissue specific localization patterns over the course of the moss life cycle. It is hoped that this foundation of knowledge of the cold shock domain will lead to a better understanding of its functional importance in higher plants of agronomic importance.The amplPCR amplification of cDNA template was accomplished by using the Universal Taqman PCR Master Mix according to suppliera#39;s instructions with an Applied Biosystems 7500 Real-Time PCR System (Branchburg, NJ, USA).


Title:Composition, Conservation, Evolution, and Function of the Cold Shock Domain Proteins in Plants
Author: Kari Beth Thompson
Publisher:ProQuest - 2008
ISBN-13:

You must register with us as either a Registered User before you can Download this Book. You'll be greeted by a simple sign-up page.

Once you have finished the sign-up process, you will be redirected to your download Book page.

How it works:
  • 1. Register a free 1 month Trial Account.
  • 2. Download as many books as you like (Personal use)
  • 3. Cancel the membership at any time if not satisfied.


Click button below to register and download Ebook
Privacy Policy | Contact | DMCA